REAL

Role of Altered Expression of miR-146a, miR-155, and miR-122 in Pediatric Patients with Inflammatory Bowel Disease.

Béres, Nóra J and Szabó, Dolóresz and Kocsis, Dorottya and Szűcs, Dániel and Kiss, Zoltán and Müller, Katalin E and Lendvai, Gábor and Kiss, András and Arató, András and Sziksz, Erna and Vannay, Ádám and Szabó, Attila J and Veres, Gábor (2016) Role of Altered Expression of miR-146a, miR-155, and miR-122 in Pediatric Patients with Inflammatory Bowel Disease. Inflammatory bowel diseases, 22 (2). pp. 327-35. ISSN 1536-4844

[img] Text
Béres-InflammBowelDis.pdf
Restricted to Registered users only

Download (668kB)

Abstract

BACKGROUND Evidence suggests the central role of tumor necrosis factor (TNF)-α in the pathomechanism of inflammatory bowel disease (IBD); however, its effect on epigenetic factors, including small non-coding microRNAs (miRs), is less known. Our present aim was the comparative investigation of the expression of TNF-α and immune response-related miRs in children with Crohn's disease (CD) and ulcerative colitis (UC). METHODS Fresh-frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) biopsies were used to analyze the expression of miR-146a, -155, -122, and TNF-α by real-time reverse transcription polymerase chain reaction in macroscopically inflamed (CD: 12 FFPE and 24 FF; UC: 10 FF) and intact (CD: 12 FFPE; 14 FF) colonic biopsies of children with IBD and controls (16 FFPE; 23 FF). The expression of miR-146a, -155, and -122 was also determined in TNF-α-treated HT-29 colonic epithelial cells. RESULTS Increased expression of TNF-α was observed in the colonic mucosa of children with CD and UC in comparison with controls. Expression of miR-146a and -155 was higher in the inflamed mucosa of children with CD and UC than in the intact mucosa. Expression of miR-122 elevated in the macroscopically intact colonic regions of CD compared with controls and patients with UC. In HT-29 cells, TNF-α treatment increased the expression of miR-146a and -155, but not that of miR-122. CONCLUSIONS Our results showed altered expression of miR-146a, -155, and -122 in the colonic mucosa of children with IBD and in TNF-α-treated colonic epithelial cells. Our data suggest the TNF-α-related involvement of these miRs in the pathogenesis of IBD.

Item Type: Article
Subjects: R Medicine / orvostudomány > RB Pathology / patológia, kórtan
Depositing User: Elvira Rigóné Kálé
Date Deposited: 22 Sep 2016 12:10
Last Modified: 22 Sep 2016 12:11
URI: http://real.mtak.hu/id/eprint/39838

Actions (login required)

Edit Item Edit Item