Effect of concomitant radiochemotherapy on invasion potential of glioblastoma

Hutóczki, Gábor and Bognár, László and Tóth, Judit and Scholtz, Beáta and Zahuczky, Gábor and Hanzély, Zoltán and Csősz, Éva and Reményi-Puskár, Judit and Kalló, Gergő and Hortobágyi, Tibor and Klekner, Almos (2016) Effect of concomitant radiochemotherapy on invasion potential of glioblastoma. Pathology & Oncology Research, 22 (1). pp. 155-160. ISSN 1219-4956

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Glioblastoma (GBM) is the most common primary brain tumor in adults with inevitable recurrence after oncotherapy. The insufficient effect of "gold standard" temozolomide-based concomitant radiochemotherapy may be due to the inability to prevent tumor cell invasion. Peritumoral infiltration depends mainly on the interaction between extracellular matrix (ECM) components and cell membrane receptors. Changes in invasive behaviour after oncotherapy can be evaluated at the molecular level by determining the RNA expression and protein levels of the invasion-related ECM components. The expression of nineteen ECM molecules was determined at both RNA and protein levels in thirty-one GBM samples. Fifteen GBM samples originated from the first surgical procedure on patients before oncotherapy, and sixteen GBM samples were collected at the second surgery due to local recurrence after concomitant chemoirradiation. RNA expressions were measured with qRT-PCR, and protein levels were determined by quantitative analysis of Western blots. Only MMP-9 RNA transcript level was reduced (p < 0.05) whereas at protein level, eight molecules showed changes concordant with RNA expression with significant decrease in brevican only. The results suggest that concomitant radiochemotherapy does not have sufficient impact on the expression of invasion-related ECM components of glioblastoma, oncotherapy does not significantly affect its invasive behavior. To avoid the spread of tumors into the brain parenchyma, supplementation of antiproliferative treatment with anti-invasive agents may be worth consideration in oncotherapy for glioblastoma.

Item Type: Article
Uncontrolled Keywords: Extracellular matrix; Glioblastoma; Invasion; Radiochemotherapy; Temozolomide
Subjects: R Medicine / orvostudomány > RB Pathology / patológia, kórtan
R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0254 Neoplasms. Tumors. Oncology (including Cancer) / daganatok, tumorok, onkológia
R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan
Depositing User: Dr. Álmos Klekner
Date Deposited: 29 Sep 2016 12:14
Last Modified: 29 Sep 2016 12:14

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