Expression pattern of invasion-related molecules in the peritumoral brain

Klekner, Álmos and Hutóczki, Gábor and Virga, József and Reményi-Puskár, Judit and Tóth, Judit and Scholtz, Beáta and Csősz, Éva and Kalló, Gergő and Steiner, László and Hortobágyi, Tibor and Bognár, László (2015) Expression pattern of invasion-related molecules in the peritumoral brain. Clinical Neurology and Neurosurgery, 139. pp. 138-143. ISSN 0303-8467

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OBJECTIVE: The effectiveness of therapy of intracerebral neoplasms is mainly influenced by the invasive behaviour of the tumour. The peritumoral invasion depends on the interaction between the tumour cells and the extracellular matrix (ECM) of the surrounding brain. The invading tumour cells induce change in the activity of proteases, synthases and expression of ECM-components. These alterations in the peritumoral ECM are in connection to the highly different invasiveness of gliomas and metastatic brain tumours. To understand the fairly modified invasive potential of anaplastic intracerebral tumours of different origin, the effect of tumour on the peritumoral ECM and alterations of invasion related ECM components in the peritumoral brain were evaluated. METHODS: For this reason the mRNA expression of 19 invasion-related molecules by quantitative reverse transcriptase polymerase chain reaction was determined in normal brain tissue (Norm), in the peritumoral brain tissue of glioblastoma (peri-GBM) and of intracerebral adenocarcinoma metastasis (peri-Met). To evaluate the translational expression of the investigated molecules protein levels were determined by targeted proteomic methods. RESULTS: Establishing the invasion pattern of the investigated tissue samples 8 molecules showed concordant difference at mRNA and protein levels in the peri-GBM and peri-Met, 11 molecules in the peri-Met and normal brain and 12 in the peri-GBM and normal brain comparison. CONCLUSION: Our results bring some ECM molecules into focus that probably play key role in arresting tumour cell invasion around the metastatic tumour, and also in the lack of impeding tumour cell migration in case of glioblastoma.

Item Type: Article
Uncontrolled Keywords: Brain metastasis; Extracellular matrix; Gene expression; Glioblastoma; Invasion; Peritumoral brain
Subjects: R Medicine / orvostudomány > RB Pathology / patológia, kórtan
R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0254 Neoplasms. Tumors. Oncology (including Cancer) / daganatok, tumorok, onkológia
Depositing User: Dr. Álmos Klekner
Date Deposited: 29 Sep 2016 12:16
Last Modified: 29 Sep 2016 12:16

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