DIC image reconstruction using an energy minimization framework to visualize optical path length distribution.

Koos, Krisztian and Molnár, József and Kelemen, Lóránd and Tamás, Gábor and Horvath, Peter (2016) DIC image reconstruction using an energy minimization framework to visualize optical path length distribution. Scientific reports, 6. p. 30420. ISSN 2045-2322

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Abstract

Label-free microscopy techniques have numerous advantages such as low phototoxicity, simple setup and no need for fluorophores or other contrast materials. Despite their advantages, most label-free techniques cannot visualize specific cellular compartments or the location of proteins and the image formation limits quantitative evaluation. Differential interference contrast (DIC) is a qualitative microscopy technique that shows the optical path length differences within a specimen. We propose a variational framework for DIC image reconstruction. The proposed method largely outperforms state-of-the-art methods on synthetic, artificial and real tests and turns DIC microscopy into an automated high-content imaging tool. Image sets and the source code of the examined algorithms are made publicly available.

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