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Beyond initiation: Human P53 plays role in transcription elongation

Borsos, Barbara Nikolett and Huliák, Ildikó and Újfaludi, Zsuzsanna and Boros, Imre Miklós and Pankotai, Tibor (2016) Beyond initiation: Human P53 plays role in transcription elongation. In: Annual Meeting of the Hungarian Biochemical Society, 2016, Szeged.

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Abstract

The P53 tumor suppressor regulates the transcrip-tion initiation of selected genes by binding to specif-ic DNA sequences at their promoters. Here we report a novel role of P53 in transcription elongation in human cells. Upon transcription elongation block-age P53 is associated with genes, which have not been reported earlier as its direct targets. P53 could be co-immunoprecipitated with active forms of RPB1, highlighting its association with the elongat-ing RNAPII. During normal transcription cycle, P53 and RPB1 localized at distinct regions of selected non-canonical P53-target genes and this pattern of localization was changed upon transcription elonga-tion block. Additionally, transcription elongation block induced the ubiquitylation and the proteo-somal degradation of RPB1. Finally, we showed that the transcription block induced RPB1 degradation is mediated by P53. Our results reveal a novel role of P53 in human cells during transcription elongation blockage that might serve to facilitate the removal of RNAPII from DNA.

Item Type: Conference or Workshop Item (Poster)
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: Dr Pankotai Tibor
Date Deposited: 30 Sep 2016 08:47
Last Modified: 30 Sep 2016 09:24
URI: http://real.mtak.hu/id/eprint/40577

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