Dima, Norbert A. and Filip, Alina and Bencze, László Csaba and Oláh, Márk and Sátorhelyi, Péter and Vértessy, Beáta (Grolmuszné) and Poppe, László (2016) Expression and purification of recombinant phenylalanine ammonia-lyase from Petroselinum crispum. STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA, 61 (2). pp. 21-34. ISSN 1224-7154
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Abstract
In the present study the molecular cloning, expression and purification of recombinant PcPAL, with a cleavable N-terminal His-tag is described. The PcPAL gene was cloned into pET-19b vector and transformed to different E.coli host cells. The optimization of expression and purification processes provided recombinant protein with high purity in its native, tetrameric fold with a yield of 7-8 mg protein / 1 L culture. The activity of the recombinant protein was tested towards its natural substrate L-Phe, the KM, and kcat values suggesting excellent catalytic properties of the recombinant enzyme.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia T Technology / alkalmazott, műszaki tudományok > TP Chemical technology / vegyipar, vegyészeti technológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 24 Jan 2017 08:13 |
| Last Modified: | 24 Jan 2017 08:13 |
| URI: | http://real.mtak.hu/id/eprint/46153 |
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