Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Nookala, Mangadevi and Mukhopadhyay, Hirak Kumar and Sivaprakasam, Amsaveni and Balasubramanian, Brindhalakshmi and Antony, Prabhakar Xavier and Thanislass, Jacob and Srinivas, Mouttou Vivek and Pillai, Raghavan Madhusoodanan (2016) Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India. Acta Microbiologica et Immunologica Hungarica, 63 (4). pp. 411-426. ISSN 1217-8950


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The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 26 Jan 2017 07:52
Last Modified: 30 Nov 2017 00:15

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