Effects of hemin, CO<sub>2</sub>, and pH on the branching of Candida albicans filamentous forms

Jakab, Ágnes and Antal, Károly and Emri, Tamás and Boczonádi, Imre and Imre, Alexandra and Gebri, Enikő and Majoros, László and Pfliegler, Walter Péter and Szarka, Máté and Balla, György and Balla, József and Pócsi, István (2016) Effects of hemin, CO<sub>2</sub>, and pH on the branching of Candida albicans filamentous forms. Acta Microbiologica et Immunologica Hungarica, 63 (4). pp. 387-403. ISSN 1217-8950


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Morphological transitions of wild-type and oxidative stress-tolerant Candida albicans strains were followed in the RPMI-FBS culture medium at pH values and CO<sub>2</sub> levels characteristic for the anatomical niches inhabited by this opportunistic human pathogen fungus, including the oral cavity as well as the intestinal and vaginal lumens. Selected cultures were also supplemented with hemin modeling bleedings. Germination as well as elongation and branching of hyphae were monitored in the cultures using time-lapse video microscopy. Unexpectedly, branching time, which is defined as the time taken until the first branch of hypha emerges for the first time after germination, correlated well with alterations in the environmental conditions meanwhile no such correlations were found for germination time (time lasted until the appearance of the germination tube). Based on these observations, hypotheses were set up to estimate the significance of branching time in the pathogenesis of both superficial and systemic candidiases.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 26 Jan 2017 07:56
Last Modified: 31 Dec 2017 00:17

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