Optimization of desferrioxamine E production by Streptomyces parvulus

Gáll, Tamás and Lehoczki, Gábor and Gyémánt, Gyöngyi and Emri, Tamás and Szigeti, Zsuzsa M. and Balla, György and Balla, József and Pócsi, István (2016) Optimization of desferrioxamine E production by Streptomyces parvulus. Acta Microbiologica et Immunologica Hungarica, 63 (4). pp. 475-489. ISSN 1217-8950

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Abstract

Siderophores are produced by a number of microbes to capture iron with outstandingly high affinity, which property also generates biomedical and industrial interests. Desferrioxamine E (DFO-E) secreted by streptomycetes bacteria can be an ideal candidate for iron chelation therapy, which necessitates its cost-effective production for in vitro and animal studies. This study focused on the optimization of DFO-E production by Streptomyces parvulus CBS548.68. Different combinations of various carbon and nitrogen sources as well as the addition of 3-morpholinopropane-1-sulfonic acid (MOPS) markedly affected DFO-E yields, which were attributed, at least in part, to the higher biomass productions found in MOPS-supplemented cultures. In MOPS-supplemented glucose and sodium glutamate medium, DFO-E productions as high as 2,009 ± 90 mg/l of culture medium were reached. High-performance liquid chromatography analysis demonstrated that a simple two-step purification process yielded DFO-E preparations with purities of ∼97%. Matrix assisted laser desorption ionization-time of flight mass spectrometry analysis showed that purified DFO-E always contained traces of desferrioxamine D2.

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