Effect of axotomy and 17beta-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice

Barabási, B. and Csondor, A. and Martin-Pozas, T. and Sanchez, A. M. and Antalffy, Géza and Siklós, László and Párducz, Árpád and Hoyk, Zsófia (2016) Effect of axotomy and 17beta-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice. NEUROSCIENCE, 319. pp. 107-115. ISSN 0306-4522

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The objective of the study was to examine whether axotomy and 17beta-estradiol affects P2X7 receptor expression and distribution in the hypoglossal nucleus. The left hypoglossal nerve of ovariectomized mice was cut and animals received a single injection of 17beta-estradiol (25mug/100 g b. w. in 20% (2- hydroxypropyl) - beta- cyclodextrin) or vehicle one hour after axotomy. Mice were sacrificed on day 4 following surgery. The area fraction of P2X7 receptor immunoreactive structures and of CD11b immunolabeled microglia, P2X7 protein concentration, and the immunoreactivity pattern of estrogen receptor alpha / beta were analyzed on both sides of the hypoglossal nucleus. Following axotomy the area fraction of P2X7 immunoreactive neurons showed a decreasing tendency, while the area fraction of P2X7 immunolabeled microglia increased significantly on the axotomized side compared with the control side in mice injected with vehicle. In animals treated with 17beta-estradiol the decrease in area fraction of neural and the increase in area fraction of microglial P2X7 immunostaining on the axotomized side were significantly enhanced compared with animals injected with vehicle. The P2X7 immunoreactivity pattern on the control side of the nucleus remained unchanged after 17beta-estradiol injection. Semi-quantitative Western blots revealed no significant difference in P2X7 protein concentration comparing the axotomized side with the control side in either experimental group. The CD11b immunoreactive microglia area fraction increased significantly following axotomy, but was not affected by 17beta-estradiol. Neither estrogen receptor alpha, nor beta colocalized with CD11b. Our results suggest that axotomy induces cell-type specific changes in P2X7 receptor expression, which may be directly regulated by 17beta-estradiol through estrogen receptor alpha or beta in neurons, but not in activated microglia.

Item Type: Article
Subjects: R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria
Depositing User: MTMT SWORD
Date Deposited: 02 Feb 2017 14:57
Last Modified: 02 Feb 2017 14:57

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