PUFA-dependent alteration of oxidative parameters of a canine mastocytoma cell line

Schmutzler, Sandra and Bachmann, Lisa and Fuhrmann, Herbert and Schumann, Julia (2010) PUFA-dependent alteration of oxidative parameters of a canine mastocytoma cell line. Acta Veterinaria Hungarica, 58 (4). pp. 453-464. ISSN 0236-6290

Text avet.58.2010.4.6.pdf Restricted to Repository staff only until 31 December 2030. Download (383kB)

Abstract

Mast cells play a key role in the immune response. Thereby, the balance of oxidative metabolism is of importance in mast cell mediator synthesis and release. Fatty acids may modify mast cell function in several ways. In this study, we investigated the influence of polyunsaturated fatty acids (PUFAs) on oxidative parameters of a canine mastocytoma cell line. C2 cells were cultured in media supplemented with linoleic acid, arachidonic acid, alpha-linolenic acid and eicosapentaenoic acid, respectively. Production of reactive oxygen species (ROS) as well as lipid peroxides was tested. Furthermore, stressor-induced DNA damage was measured. Exposure of the cells to PUFAs resulted in a significant increase in the synthesis of both ROS and lipid peroxides. Distinct differences between the PUFAs tested underline the impact of the unsaturation degree of fatty acids as well as the position of double bonds on mast cells.

Actions (login required)

Edit Item