REAL

Isolation and characterisation of Mycobacterium avium and Rhodococcus equi from granulomatous lesions of swine lymph nodes in Slovenia

Pate, Mateja and Zdovc, Irena and Pirš, Tina and Krt, B. and Ocepek, M. (2004) Isolation and characterisation of Mycobacterium avium and Rhodococcus equi from granulomatous lesions of swine lymph nodes in Slovenia. Acta Veterinaria Hungarica, 52 (2). pp. 143-150. ISSN 0236-6290

[img] Text
avet.52.2004.2.2.pdf
Restricted to Repository staff only until 30 April 2024.

Download (156Kb)

Abstract

Granulomatous lesions in bovine and especially swine lymph nodes are still frequently observed during routine veterinary meat inspections even though Mycobacterium bovis infections are no longer detected in domestic animals in Slovenia. Different lymph nodes of pigs (n = 260) were investigated using classical bacteriological and molecular methods. Mycobacterium avium alone was isolated in 47.3% of pigs and in mixed infection with Rhodococcus equi in 3.9% of pigs. R. equi alone was isolated in 27.3% and in mixed infection with mycobacteria other than M. avium in 1.5% of pigs. A total of 133 M. avium isolates were typed using the IS1245, IS901 and FR300 PCR. Almost two thirds (60.9%) of isolates belonged to M. avium hominissuis (IS901-, IS1245+ genotype), 33.8% of isolates belonged to M. avium avium (IS901+, IS1245+ genotype) and 5.3% of isolates remained non-typed. Fifty out of 85 R. equi isolates were tested for the virulence-associated antigens (VapA and VapB). Nearly two thirds (60.0%) were positive for VapB while all the other isolates were VapA- and VapB-negative.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: Katalin Barta
Date Deposited: 15 Feb 2017 13:08
Last Modified: 15 Feb 2017 13:08
URI: http://real.mtak.hu/id/eprint/49065

Actions (login required)

View Item View Item