High prevalence of NDM metallo-β-lactamase among ESBL-producing Escherichia coli İsolates

Çetinkol, Yeliz and Sandalli, Cemal and Çalgin, Mustafa Kerem and Yildirim, Arzu Altunçekiç and Akyildiz, Esma and Karaman, Esin and Çiçek, Ayşegül Çopur (2017) High prevalence of NDM metallo-β-lactamase among ESBL-producing Escherichia coli İsolates. Acta Microbiologica et Immunologica Hungarica, 64 (2). pp. 131-141. ISSN 1217-8950


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Resistance to β-lactams in Enterobacteriaceae has been increasing worldwide. This study aimed to determine the frequency of β-lactamase genes and antibiotic resistance rates of 140 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates obtained from urinary tract infection in Ordu Province, Turkey. Isolates were identified by classic methods and by automated system. ESBL production was confirmed by double disk synergy test and antimicrobial susceptibility was investigated by disk diffusion method. All isolates were screened for β-lactamase coding genes from three groups (A, B, and D) by polymerase chain reaction. The highest rate of susceptible isolates was observed for imipenem (IPM, 99.3%) and ertapenem (ETP, 97.9%), and the highest rate of resistant isolates was observed for cefuroxime (97.9%), ceftriaxone (97.2%), and cefazolin (90.7%). In our study, bla<sub>CTX-M1-like</sub> group was the most prevalent β-lactamase (n = 109), followed by bla<sub>TEM</sub> (n = 68), bla<sub>CTX-M2 </sub>(n = 22), and bla<sub>SHV</sub> (n = 2). By contrast to low resistance rate to IPM and ETP, we determined bla<sub>NDM</sub> in 31 isolates (22.1%). In co-prevalence of bla<sub>NDM-1</sub> and ESBL-coding genes, a low carbapenem resistance was determined. We can confirm that bla<sub>CTX-M1-types</sub> are still the most frequent β-lactamase coding gene in Turkey. Our study showed the highest prevalence of bla<sub>NDM-1</sub> metallo-β-lactamase coding gene in ESBL-producing E. coli.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 13 Jul 2017 08:27
Last Modified: 30 Apr 2018 23:15

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