Preparation of protein loaded chitosan microparticles by combined precipitation and spherical agglomeration

Fodorné Kardos, Andrea and Tóth, Judit and Gyenis, János (2013) Preparation of protein loaded chitosan microparticles by combined precipitation and spherical agglomeration. POWDER TECHNOLOGY, 244. pp. 16-25. ISSN 0032-5910

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Combined precipitation and spherical agglomeration was carried out in the non-miscible region of ethyl acetate-ethanol-water ternary solvent system. At first, w/o type quasi emulsion was prepared by sequential introduction of aqueous solutions of human serum albumin (HSA), chitosan (CS), and poly(4-styrenesulfonate) (PSS) into an ethyl acetate-ethanol solvent mixture. HSA was used to model a protein type drug, while CS and PSS served as matrix material in the obtained composite particles. PSS also served as chemical precipitation agent for both of the HSA and CS. The solubility of all these substances was reduced by introduction of additional amounts of ethyl acetate-ethanol mixture and/or ethanol as poor solvents. Due to the counter-diffusion of the good and poor solvents between the water rich droplets and the ethyl acetate-ethanol rich continuous phase, the aqueous phase gradually disappeared and partial agglomeration of the precipitated solids and their transfer to the continuous organic phase took place. The paper gives a report on the effect of several process variables on the quality of the obtained microparticles, such as their shape and stability against disintegration. The effects of the composition of the ternary solvent mixture, the route of its variation, the feeding method and composition of the added poor solvents, the stirring rate and the duration of agitation were studied.

Item Type: Article
Uncontrolled Keywords: solvent effect; process technology; Precipitation; Phase separation; particle size; microencapsulation; microemulsion; drug delivery system; CRYSTALLIZATION; composite material; chemical composition; ARTICLE; aqueous solution; WATER; polystyrenesulfonic acid; human serum albumin; ALCOHOL; acetic acid ethyl ester; Organic solvents; SPHERES; Solvents; PROTEINS; Precipitation (chemical); MIXTURES; LASERS; Ethanol; Emulsification; composite materials; Chitosan; AGGLOMERATION; Ternary solvent systems; Ternary solvent mixture; Poly(4-styrenesulfonate); Composite particles; Chitosan microparticles; Anti-solvent precipitations; Spherical agglomeration; HSA/chitosan composite particles; controlled drug release; Chemical Precipitation; Antisolvent precipitation
Subjects: Q Science / természettudomány > QD Chemistry / kémia
Depositing User: MTMT SWORD
Date Deposited: 23 Jul 2013 11:41
Last Modified: 23 Jul 2013 11:41

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