RT-PCR detection of the expression of COP1 and SINAT5 E3 ubiquitin ligase genes in different organs of Zea mays L.

Gholizadeh, A. and Kohnehrouz, B. (2011) RT-PCR detection of the expression of COP1 and SINAT5 E3 ubiquitin ligase genes in different organs of Zea mays L. Cereal Research Communications, 39 (1). pp. 1-11. ISSN 0133-3720

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Abstract

Using bioinformatic data, we found that maize homologues of the Arabidopsis single subunit RING type ubiquitin ligase genes, COP1 (Constitutive Photomorphogenesis 1) and SINAT5 (Seven in Absentia in Arabidopsis thaliana 5) have been frequently predicted during large-scale cDNA sequencing project and released to databases since 2008. Despite this general information, no tissue specific expression profiles of these genes were published to our knowledge so far. In the present research, the expression and the relative levels of COP1 and SINAT5 transcripts were detected using reverse transcription and polymerase chain reaction (RT-PCR) with template materials collected separately from three different organs (root, leaf and seed) of maize plants grown under the normal growth conditions. The results not only confirmed the presence of COP1 transcript in all three test samples but also revealed its abundance in root tissues that may be consistent to its large number of targets for ubiquitination in darkness. Analysis of SINAT5 expression profile revealed a detectable band in leaf tissue sample that may be related to its specific roles in this organ.

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