Identification of Fusarium species by isozyme analysis

Láday, M. and Szécsi, Á. (2002) Identification of Fusarium species by isozyme analysis. Acta Microbiologica et Immunologica Hungarica, 49 (2-3). pp. 321-330. ISSN 1217-8950

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Cellulose-acetate electrophoresis (CAE) was used to investigate isozyme polymorphisms among different isolates of Fusarium cerealis, F. culmorum, F. graminearum and F. pseudograminearum. After initial testing of 18 enzymes in three buffer systems for activity and resolution of bands, 12 proved to be appropriate for analysis of the full sample set. Comparing the different electrophoretic types (ETs), adenylate kinase (AK), NADP dependent glutamate dehydrogenase (NADP GDH), peptidase B (PEP B), peptidase D (PEP D) and phosphoglucomutase (PGM) proved to be diagnostic for at least one species examined. However, only PEP D was useful alone as a marker to distinguish the four taxa studied providing a rapid and simple CAE based diagnostic protocol.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: xFruzsina xPataki
Date Deposited: 11 Sep 2017 06:59
Last Modified: 11 Sep 2017 06:59

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