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Molecular Microbiology of Gut Bacteria: Genetic Diversity and Community Structure Analysis

Peterka, M. and Tepšič, Katarina and Accetto, T. and Kostanjšek, R. and Ramšak, Andreja and Lipoglavšek, L. and Avguštin, G. (2003) Molecular Microbiology of Gut Bacteria: Genetic Diversity and Community Structure Analysis. Acta Microbiologica et Immunologica Hungarica, 50 (4). pp. 395-406. ISSN 1217-8950

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Abstract

Recently developed molecular biology approaches make possible the detailed genetic, taxonomic and ecological examination of microorganisms from various habitats. Animal gut represents one of the most complex microbial ecosystems with a large degree of microbial biodiversity present. Bacteria inhabiting the gut usually play important roles in metabolic transformations of substrates and sometimes, e.g. in ruminants, they make the basis for an obligate symbiosis with the host. Here we discuss molecular microbiology as a strategy for examination of gut bacteria, concentrating on a typical and in such environment dominant group of strictly anaerobic Gram-negative bacteria from the phylogenetic group Cytophaga/Flexibacter/Bacteroides. The bacteria from the genus Prevotella are the most abundant Gram-negative bacteria in the rumen and form a distinctive phylogenetic cluster, clearly separated from prevotellas isolated from other ecological niches. They may represent a good choice for a model organism in genetic manipulation experiments and for studies of gene transfer mechanisms taking place in the gut. The molecular tools for detection and monitoring of ruminal prevotellas are discussed.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: xFruzsina xPataki
Date Deposited: 11 Sep 2017 18:18
Last Modified: 30 Nov 2023 00:15
URI: http://real.mtak.hu/id/eprint/62231

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