Mycobacterium bovis BCG induces high mobility group box 1 protein release from monocytic cells

Hofner, Peter (2007) Mycobacterium bovis BCG induces high mobility group box 1 protein release from monocytic cells. Acta Microbiologica et Immunologica Hungarica, 54 (4). pp. 323-337. ISSN 1217-8950

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Abstract

High mobility group box 1 protein (HMGB-1), a nuclear protein is a critical cytokine that mediates the response to infection, injury and inflammation.The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB-1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB-1 from supernatants of cells, following induction with LPS, Staphylococcus aureus , and Mycobacterium bovis BCG. HMGB-1 was subjected to MALDI-TOF mass and PSD analysis. Quantitation of the secreted HMGB-1 was performed by ELISA. The BCG strain induced higher amounts of secreted HMGB-1 than LPS or Staphylococcus aureus . The translocation of the HMGB-1 to the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations.Conclusion: Our pilot experiments draw attention the to HMGB-1-inducing ability of Mycobacterium bovis . Assessment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.

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