Use of PCR based RAPD technique for characterization of Chaetomium globosum isolates

Ahammed, S. K. and Aggarwal, R. and Renu, (2005) Use of PCR based RAPD technique for characterization of Chaetomium globosum isolates. Acta Phytopathologica et Entomologica Hungarica, 40 (3-4). pp. 303-314. ISSN 0238-1249

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Chaetomium globosum Kunze Ex. Fries has been identified as a potential antagonist of Bipolaris sorokiniana and Ascochyta rabiei. Nine isolates of this biocontrol agent were characterized based on RAPD technique using 40 random primers of OPB and OPM series, out of which 24 primers gave a total of 356 amplification products, showing 91% polymorphism. RAPD analysis revealed that C. globosum isolates taken under study formed genetically two distinct clusters, with isolates Cg2, Cg3 and Cg4 in one cluster and the remaining isolates in another cluster, which in turn was grouped separately into two subclusters separating isolates Cg6 and Cg8 from Cg1, Cg5, Cg7 and Cg9. Similarity matrix indicated that Cg1 and Cg2 were genetically distinct as they showed only 29.4% similarity followed by Cg2 and Cg7 showing 31.1% similarity, while isolates Cg7 and Cg9 were found to be genetically similar with 68.9% similarity index.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH540 Ecology / ökológia
Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan
Q Science / természettudomány > QL Zoology / állattan > QL01 Systematic zoology / állatrendszertan
Depositing User: xBarbara xBodnár
Date Deposited: 26 Oct 2017 06:25
Last Modified: 26 Oct 2017 06:25

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