Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp.

Prasanna, Radha and Wattal Dhar, Dolly and Dominic, T. K. and Tiwari, O. N. and Singh, P. K. (2003) Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp. Acta Biologica Hungarica, 54 (1). pp. 113-120. ISSN 0236-5383

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Abstract

Chemical (N′ methyl-N′-nitro-N-nitrosoguanidine) mutagenesis and penicillin selection were utilised to isolate a phenotypically altered mutant of cyanobacterium Synechocystis sp. This mutant (ntm60A) exhibited enhanced protein content and nitrogen fixing potential but lower amount of chlorophyll and nitrate reductase activity. A remarkable and significant increase was observed in the total phycobiliprotein content of the mutant, especially in relation to the amount of phycoerythrin. This strain can be exploited as a rich source of natural coloring agent such as phycobilins in the bioindustry.

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