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Quantitative evaluation of macrophage phagocytosing capacity by a fluorometric assay

Miliukiené, Valé and Biziuleviciené, Gené and Pilinkiené, Audroné (2003) Quantitative evaluation of macrophage phagocytosing capacity by a fluorometric assay. Acta Biologica Hungarica, 54 (3-4). pp. 347-356. ISSN 0236-5383

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Abstract

This paper reviews sensitive and simple quantitative evaluation of macrophage phagocytosing capacity by applying fluorescein-labeled Sacharomyces cerevisiae cells. Yeast cells were conjugated with fluoresceinisothiocyanate (FITC) and used as fluorescent particles. A time course analysis within this method showed that phagocytosis of yeast cells was temperature dependent and that the number of that ones ingested by macrophages increased rapidly during the initial 60 min of incubation at 37 °C. Free fluorescent cells can be effectively removed by aspiration from the well. Furthermore, yeast cells required preopsonization with serum to achieve optimal uptake of the cells. The uptake of nonopsonized yeast cells by macrophages was significantly lower than that of opsonized cells (P<0.05). We propose that about 50% of mouse macrophages can carry functionally active FcR responsible for phagocytosis.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 13 Nov 2017 15:48
Last Modified: 13 Nov 2017 15:48
URI: http://real.mtak.hu/id/eprint/69831

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