REAL

A comparative methodical study of the faecal steroid analysis on birds: looking for a valid method of testosterone determination

Kelemen, K. and Péczely, P. and Szőke, Zs. and Ladjánszky, V. (2003) A comparative methodical study of the faecal steroid analysis on birds: looking for a valid method of testosterone determination. Acta Biologica Hungarica, 54 (3-4). pp. 285-298. ISSN 0236-5383

[img] Text
abiol.54.2003.3-4.7.pdf
Restricted to Repository staff only until 31 December 2023.

Download (470kB)

Abstract

In a comparative study, a relatively simple and high sensitivity method was developed for analysis of testosterone-equivalent(s) in the faeces of different bird species. To determine the recovery of extractions and purifications, tritium-labelled testosterone was added to the wet samples. Then the samples were treated with sodium dodecil sulphate (SDS), an emulsificator to “open-up” the complex, lipid-coated particles of faecal samples. This emulsification resulted in the decrease of the quantity of interfering substances after diethyl-ether extraction and the linearity of the measured testosterone equivalents from aliquots in the range of 2 and 10 mg of faeces. In the RIA, we applied a group specific polyclonal testosterone antibody which cross-reacted with reduced metabolites and at a certain level with sulphate conjugates as well. The use of Helix enzymes did not modified significantly the results of the analysis relating to a low level of conjugated androgens in the faecal extracts. The biological validity of the method was tested on domestic cockerels, where between the plasma and faecal testosterone values a four hours phase shift was observed, with a correlation of 0.6355. This method is suitable for “non invasive”, behavioural-ethological studies.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 13 Nov 2017 15:59
Last Modified: 13 Nov 2017 15:59
URI: http://real.mtak.hu/id/eprint/69842

Actions (login required)

Edit Item Edit Item