A propolis extract and the labeling of blood constituents with technetium-99m

Jesus, L. M. and Abreu, P. R. C. and C. Almeida, Marcela and C. Brito, Lavínia and F. Soares, Sheila and De Souza, D. E. and C. Bernardo, Luciana and Fonseca, A. S. and Bernardo-Filho, M. (2006) A propolis extract and the labeling of blood constituents with technetium-99m. Acta Biologica Hungarica, 57 (2). pp. 191-200. ISSN 0236-5383

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Abstract

Since ancient times propolis has been employed for many human purposes because to their favourable properties. Blood constituents labeled with technetium-99m ( 99m Tc) have been used in nuclear medicine procedures. Some authors have reported that synthetic or natural drugs can interfere with the labeling of blood constituents with 99m Tc. The aim of this work was to evaluate the action of a propolis extract on the labeling of blood elements with 99m Tc. Samples of whole blood of male Wistar rats were incubated in sequence with an aqueous propolis extract at different concentrations, stannous chloride and 99m Tc, as sodium pertechnetate. Blood samples were centrifuged to separate plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were also separated after precipitation in trichloroacetic acid solution and centrifugation. The radioactivity was counted and the percentage of incorporated radioactivity (%ATI) for each fraction was calculated. The data obtained showed that the aqueous propo- lis extract used decreased significantly the %ATI in plasma proteins at higher concentration studied. Results suggest that at high concentration the constituents of this extract could alter the labeling of plas- ma proteins competing with same binding sites of the 99m Tc on the plasma proteins or acting as antioxi- dant compounds.

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