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In vitro effect of pesticides (dichlofluanid, endosulfan, simazine, tolylfluanid and triallate) on proliferative activity of animal derived cell cultures

Holovská, Vanda and Pistl, J. and Kovalkovičová, Natália (2007) In vitro effect of pesticides (dichlofluanid, endosulfan, simazine, tolylfluanid and triallate) on proliferative activity of animal derived cell cultures. Acta Biologica Hungarica, 58 (1). pp. 61-74. ISSN 0236-5383

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Abstract

In this study pesticides with different chemical structures (dichlofluanid, endosulfan, simazine, tolylflu- anid and triallate) were examined for their potential cytotoxic effect on proliferative activity of cell cul- tures of mammalian origin. Cell lines Madin-Darby Bovine Kidney (MDBK), Rabbit Kidney (RK13), Porcine Kidney (PK15), and semicontinual line of Bovine Embryonic Pulmonary Cells (BEPC) were used in the study. From these cell cultures cell proliferative activity was suppressed most intensively in PK15 culture by endosulfan (10 –1 –10 –6 M). The least effect on cell proliferation in all cell cultures test- ed, with the exception PK 15 (10 –1 –10 –2 M), was recorded after simazine exposure. On the basis of IC 50 values the cytotoxic effect was: dichlofluanid (IC 50 = 10 –3.94 M) > tolylfluanid (IC 50 =10 –3.69 M) > endo- sulfan (IC 50 =10 –3.24 M) > triallate (IC 50 =10 –3.12 M) > simazine (IC 50 =10 –1.78 M). The comparison of average IC 50 values of cell cultures revealed that the most sensitive cell lines were PK15 (IC 50 =10 –3.27 M) and RK13 (IC 50 =10 –3.21 M), whereas MDBK (IC 50 =10 –2.55 M) and BEPC (IC 50 =10 –2.52 M) were less sensitive to pesticide exposure.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 20 Nov 2017 14:57
Last Modified: 20 Nov 2017 14:57
URI: http://real.mtak.hu/id/eprint/70111

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