Expression of two metallothionein genes in different brain regions of common carp

Ali, K. and Ferencz, Ágnes and Deér, Aranka and Nemcsók, J. and Hermesz, Edit (2009) Expression of two metallothionein genes in different brain regions of common carp. Acta Biologica Hungarica, 60 (2). pp. 149-158. ISSN 0236-5383

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The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd2+ was investigated in the brain of common carp (Cyprinus carpio), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain regionand stressor-specific. Cd2+ affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially; MT-2 was induced significantly more strongly in the olfactory lobe than in the cerebellum or midbrain. A sudden temperature drop mainly affected the expression of the MT-1 gene; after 5 h of cold shock, the MT-1 mRNA level was about 25% of the basal value in the cerebellum and the midbrain region. The MT-2 expression did not change significantly during this treatment.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 23 Nov 2017 14:35
Last Modified: 23 Nov 2017 14:35

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