Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression

Nagymihály, Marianna and Veluchamy, Alaguraj and Györgypál, Zoltán and Ariel, Federico and Jégu, Teddy and Szűcs, Attila and Kereszt, Attila and Kondorosi, Éva (2017) Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114 (17). pp. 4543-4548. ISSN 0027-8424


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The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodule-specific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes' active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplication-dependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.

Item Type: Article
Uncontrolled Keywords: TRANSCRIPTION; ENDOREDUPLICATION; PLANT; Chromatin; DIFFERENTIATION; ARABIDOPSIS; ROOT-NODULES; MEDICAGO-TRUNCATULA; genome-wide analysis; REVEALS DNA METHYLATION; Chromatin structure; DNA methylation; nodule-specific cysteine-rich peptides; symbiosis; Medicago truncatula
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Depositing User: MTMT SWORD
Date Deposited: 23 Jan 2018 09:59
Last Modified: 23 Jan 2018 09:59

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