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Factors influencing algal photobiohydrogen production in algal-bacterial co-cultures

Lakatos, Gergely and Balogh, Daniella and Farkas, Attila and Ördög, Vince and Nagy, Péter Tamás and Bíró, Tibor and Maróti, Gergely (2017) Factors influencing algal photobiohydrogen production in algal-bacterial co-cultures. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28. pp. 161-171. ISSN 2211-9264

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Abstract

Algal-bacterial co-cultures represent an alternative way for algal biohydrogen generation. Efficient algal hydrogen production requires anaerobiosis and electrons accessible for the algal FeFe-hydrogenases. A number of factors strongly influence the development of this optimal environment. Various algal strains were tested for hydrogen evolution with a selected bacterial partner, a fully hydrogenase deficient Escherichia coli. During the hunt for the most efficient algae strains, gas-to-liquid phase ratio, algal optical density and algal cell size were identified as crucial factors influencing algal hydrogen evolution rate, accumulated algal hydrogen yield, carbon dioxide and oxygen levels as well as acetic acid consumption in illuminated algal-bacterial cultures. The highest accumulated hydrogen yields were observed for the different algal partners under similar experimental setup. The combination of a gas-to-liquid phase ratio of 1/1 with an algae cell density of 3.96 * 10(8) algae cell ml(-1) (OD750:1) resulted in the highest accumulated algal hydrogen yields under continuous illumination of similar to 50 mu mol m(-2) s(-1) light at 25 degrees C irrespective of the applied algae strain. Accumulated hydrogen yield was also strongly influenced by the algal cell size, smaller cell size correlated with higher hydrogen evolution rate. The highest accumulated algal hydrogen yield (88.98 +/- 2.19 ml H-2 l(-1) d(-1)) was obtained with Chlorella sp. MACC 360 -E. coli Delta hypF co-culture.

Item Type: Article
Uncontrolled Keywords: PYRENOIDOSA; PHOTOBIOREACTORS; H-2; PHOTOPRODUCTION; LIGHT-INTENSITY; NITROGEN LIMITATION; CHLORELLA-PROTOTHECOIDES; SULFUR DEPRIVATION; HYDROGEN-PRODUCTION; DEPRIVED CHLAMYDOMONAS-REINHARDTII; Cell Size; green algae; PHOTOSYNTHESIS; Biohydrogen; Algal bacterial co-culture
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 24 Jan 2018 09:37
Last Modified: 24 Jan 2018 09:37
URI: http://real.mtak.hu/id/eprint/73257

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