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The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones

Szikora, Szilárd and Földi, István and Tóth, Krisztina and Migh, Ede and Leipold Vig, Andrea and Bugyi, Beáta and Maléth, József and Hegyi, Péter and Kaltenecker, Péter and Mihály, József (2017) The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones. JOURNAL OF CELL SCIENCE, 130 (15). pp. 2506-2519. ISSN 0021-9533

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Abstract

Directed axonal growth depends on proper coordination of the actin and microtubule cytoskeleton in the growth cone. However, despite the relatively large number of proteins implicated in actin-microtubule cross-talk, the mechanisms whereby actin polymerization is coupled to microtubule stabilization and advancement in the peripheral growth cone remained largely unclear. Here we identified the formin DAAM as a novel factor playing a role in concerted regulation of actin and microtubule remodeling in Drosophila primary neurons. In vitro DAAM binds to F-actin as well as microtubules and it has the ability to crosslink the two filament systems. Accordingly, DAAM associates with the neuronal cytoskeleton, and a significant fraction of DAAM accumulates at places where the actin filaments overlap with that of microtubules. Loss of DAAM affects growth cone and microtubule morphology and several aspects of microtubule dynamics, whereas biochemical and cellular assays revealed a microtubule stabilization activity and binding to the microtubule tip protein EB1. Together these data suggest that besides operating as an actin assembly factor, DAAM is involved in linking filopodial actin remodeling to microtubule stabilization during axonal growth.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 29 Jan 2018 15:26
Last Modified: 29 Jan 2018 15:26
URI: http://real.mtak.hu/id/eprint/73488

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