Varga, Andrea and Bata, Zsófia and Csuka, Pál and Bordea, Diana Monica and G. Vértessy, Beáta and Irimie, Florin Dan and Poppe, László and Bencze, László Csaba (2017) A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS. STUDIA UNIVERSITATIS BABES-BOLYAI SERIES CHEMIA, 62 (3). pp. 293-308. ISSN 0039-3401
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Abstract
This study describes cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E.coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD04 Organic chemistry / szerves kémia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 27 Feb 2018 15:14 |
| Last Modified: | 27 Feb 2018 15:14 |
| URI: | http://real.mtak.hu/id/eprint/75166 |
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