Evaluation of commonly used tear sampling methods and their relevance in subsequent biochemical analysis

Rentka, Anikó and Köröskényi, Krisztina and Hársfalvi, Jolán and Szekanecz, Zoltán and Szűcs, Gabriella and Kemény-Beke, Ádám (2017) Evaluation of commonly used tear sampling methods and their relevance in subsequent biochemical analysis. ANNALS OF CLINICAL BIOCHEMISTRY, 54 (5). pp. 521-529. ISSN 0004-5632

[img] Text
Restricted to Registered users only

Download (184kB) | Request a copy


The human precorneal tear film (PCTF) is a special body fluid, since it is a complex mixture of proteins, lipids small bioactive molecules and their concentrations and relative distribution represent not only the metabolic state of the ocular surface but also the systemic and local homeostasis of the outer eye and the human body. This suggests that biochemical analysis of the PCTF composition may provide a non-invasive tool for diagnosis and monitoring of disease progression or treatment efficacy in human medicine. However, collecting tears is demanding, and obtaining reproducible and unaltered samples is challenging because of the small sample volumes of tears. Several methods are available for tear collection as a preparatory step of PCTF analysis, and the collection method used has to be assessed since it has a critical impact on the effectiveness of the assays and on the quality of the results. Each sampling method has advantages and disadvantages, therefore it is not easy to choose the appropriate collecting method for tear collection. To overcome these limitations various methods have been recommended by different authors for special aspects of specific tests. The aim of our review was to evaluate tear sampling methods with regard to our ongoing biochemical analysis.

Item Type: Article
Uncontrolled Keywords: tear diagnostics; Bioactive components; tear collection; tear sampling; precorneal tear film
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Depositing User: MTMT SWORD
Date Deposited: 10 May 2018 13:05
Last Modified: 10 May 2018 13:05

Actions (login required)

Edit Item Edit Item