Molecular mechanisms of developmentally programmed crinophagy in Drosophila

Csizmadia, Tamás and Lőrincz, Péter and Hegedűs, Krisztina and Széplaki, Szilvia and Lőw, Péter and Juhász, Gábor (2018) Molecular mechanisms of developmentally programmed crinophagy in Drosophila. Journal of Cell Biology, 217 (1). pp. 361-374. ISSN 0021-9525


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At the onset of metamorphosis, Drosophila salivary gland cells undergo a burst of glue granule secretion to attach the forming pupa to a solid surface. Here, we show that excess granules evading exocytosis are degraded via direct fusion with lysosomes, a secretory granule-specific autophagic process known as crinophagy. We find that the tethering complex HOPS (homotypic fusion and protein sorting); the small GTPases Rab2, Rab7, and its effector, PLEKHM1; and a SNAP receptor complex consisting of Syntaxin 13, Snap29, and Vamp7 are all required for the fusion of secretory granules with lysosomes. Proper glue degradation within lysosomes also requires the Uvrag-containing Vps34 lipid kinase complex and the v-ATPase proton pump, whereas Atg genes involved in macroautophagy are dispensable for crinophagy. Our work establishes the molecular mechanism of developmentally programmed crinophagy in Drosophila and paves the way for analyzing this process in metazoans.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Depositing User: MTMT SWORD
Date Deposited: 18 May 2018 11:09
Last Modified: 18 May 2018 11:09

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