Development and characterization of ultra-porous silica films made by the sol–gel method. Application to biosensing

Desfours, Caroline and Calas-Etienne, Sylvie and Horváth, Róbert and Martin, Marta and Gergely, Csilla (2013) Development and characterization of ultra-porous silica films made by the sol–gel method. Application to biosensing. APPLIED PHYSICS A - MATERIALS SCIENCE AND PROCESSING. pp. 1-9. ISSN 0947-8396

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Abstract

The aim of this work is to demonstrate the sensing ability of reverse-symmetry waveguides to investigate adsorption of casein and build-up of poly-L-lysine mediated casein multilayers. A first part of this study is dedicated to the elaboration and characterization of ultra-porous thin films with very low refractive indices by an appropriate sol–gel method. This will form the basis of our planar optical sensors. Optical waveguide light mode spectroscopy is a real-time and sensitive method to study protein adsorption kinetics and lipid bilayers. We used it to test the obtained waveguides for in-situ monitoring of biomolecule adsorption. As a result, significant changes in the incoupling peak position were observed during the layer-by-layer adsorption. Finally, refractive index and thickness of the adsorbed layers were established.

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