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Endocannabinoid Tone Regulates Human Sebocyte Biology

Zákány, Nóra and Oláh, Attila and Markovics, Arnold and Takács, Erika and Aranyász, Andrea and Nicolussi, Simon and Piscitelli, Fabiana and Allara, Marco and Pór, Ágnes and Kovács, Ilona and Zouboulis, Christos C. and Gertsch, Jürg and Di Marzo, Vincenzo and Bíró, Tamás and Szabó, Tamás (2018) Endocannabinoid Tone Regulates Human Sebocyte Biology. Journal of Investigative Dermatology. ISSN 0022-202X

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Abstract

We have previously shown that endocannabinoids (eCBs)(e.g., anandamide) are involved in the maintenance of homeostatic sebaceous lipid production inhuman sebaceous glands and thateCB treatment dramatically increases sebaceous lipid production. Here, we aimed to investigate the expression of the major eCB synthesizing and degrading enzymes and to study the effects of eCB uptake inhibitors on human SZ95 sebocytes, thus exploring the role of the putative eCB membrane transporter, which has been hypothesized to facilitate the cellular uptake and subsequent degradation of eCBs. We found that the major eCB synthesizing (N-acyl phosphatidylethanolaminespecific phospholipase D, and diacylglycerol lipase-a and -b) and degrading (fatty acid amide hydrolase, monoacylglycerol lipase) enzymes are expressed in SZ95 sebocytes and also in sebaceous glands (except for diacylglycerol lipase-a, the staining of whichwas dubious in histological preparations). eCB uptake-inhibitionwith VDM11 induced amoderate increase insebaceous lipid production and also elevated the levels of variouseCBs and related acylethanolamides. Finally, we found that VDM11 was able to interfere with the proinflammatory action of the TLR4 activator lipopolysaccharide. Collectively, our data suggest that inhibition of eCB uptake exerts anti-inflammatory actions and elevates both sebaceous lipid production and eCB levels; thus, these inhibitors might be beneficial in cutaneous inflammatory conditions accompanied by dry skin.

Item Type: Article
Subjects: Q Science / természettudomány > QP Physiology / élettan
R Medicine / orvostudomány > RL Dermatology / bőrgyógyászat
Depositing User: Dr. Attila Oláh
Date Deposited: 28 Sep 2018 10:07
Last Modified: 28 Sep 2018 10:07
URI: http://real.mtak.hu/id/eprint/85796

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