Microtubule organization in presynaptic boutons relies on the formin DAAM

Migh, Ede and Gotz, T. and Földi, István and Szikora, Szilárd and Gombos, Rita I. and Darula, Zsuzsanna and Medzihradszky F., Katalin and Maléth, József and Hegyi, Péter and Mihály, József (2018) Microtubule organization in presynaptic boutons relies on the formin DAAM. DEVELOPMENT, 145 (6). pp. 1-13. ISSN 0950-1991


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Regulation of the cytoskeleton is fundamental to the development and functioning of synaptic terminals, such as neuromuscular junctions. Nevertheless, despite identification of numerous proteins that regulate synaptic actin and microtubule dynamics, the mechanisms of cytoskeletal control during terminal arbor formation has remained largely elusive. Here, we show that DAAM, a member of the formin family of cytoskeleton organizing factors, is an important presynaptic regulator of neuromuscular junction development in Drosophila We demonstrate that the actin filament assembly activity of DAAM plays a negligible role in terminal formation; rather, DAAM is necessary for synaptic microtubule organization. Genetic interaction studies consistently link DAAM with the Wg/Ank2/Futsch module of microtubule regulation and bouton formation. Finally, we provide evidence that DAAM is tightly associated with the synaptic active zone scaffold, and electrophysiological data point to a role in the modulation of synaptic vesicle release. Based on these results, we propose that DAAM is an important cytoskeletal effector element of the Wg/Ank2 pathway involved in the determination of basic synaptic structures, and, additionally, DAAM may couple the active zone scaffold to the presynaptic cytoskeleton.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Depositing User: MTMT SWORD
Date Deposited: 31 Jan 2019 10:14
Last Modified: 31 Jan 2019 10:14

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