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Calibration of rotation axes for multi-axis goniometers in macromolecular crystallography

White, Ian K. and Bugris, Valéria and McCarthy, Andrew A. and Ravelli, Raimond B. G. and Csankó, Krisztián and Cassett, Alberto and Brockhauser, Sándor (2018) Calibration of rotation axes for multi-axis goniometers in macromolecular crystallography. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 51 (5). pp. 1421-1427. ISSN 0021-8898

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Abstract

The installation of multi-axis goniometers such as the ESRF/EMBL miniKappa goniometer system has allowed the increased use of sample reorientation in macromolecular crystallography. Old and newly appearing data collection methods require precision and accuracy in crystal reorientation. The proper use of such multi-axis systems has necessitated the development of rapid and easy to perform methods for establishing and evaluating device calibration. A new diffraction-based method meeting these criteria has been developed for the alibration of the motors responsible for rotational motion. This method takes advantage of crystal symmetry by comparing the orientations of a sample rotated about a given axis and checking that the magnitude of the real rotation fits the calculated angle between these two orientations. Hence, the accuracy and precision of rotational motion can be assessed. This rotation calibration procedure has been performed on several beamlines at the ESRF and other synchrotrons. Some resulting data are presented here for reference. © 2018, Wiley-Blackwell. All Rights Reserved.

Item Type: Article
Uncontrolled Keywords: CALIBRATION; CALIBRATION; ROTATION; MACROMOLECULES; Crystallography; Crystal symmetry; Crystal orientation; Accuracy and precision; REORIENTATION; REORIENTATION; Goniometers; Calibration procedure; MACROMOLECULAR CRYSTALLOGRAPHY; MACROMOLECULAR CRYSTALLOGRAPHY; Kappa diffractometer; Rotational flow; Crystal reorientation; Data collection method; Device calibration; Rotational motion;
Subjects: Q Science / természettudomány > QD Chemistry / kémia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 01 Feb 2019 09:06
Last Modified: 01 Feb 2019 09:06
URI: http://real.mtak.hu/id/eprint/90980

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