Production and evaluation of leukocyte- and thrombocyte-rich fibrin membranes in birds

Fernandes, Laís L. and Rahal, Sheila C. and Fabro, Alexandre T. and Batah, Sabrina S. and Hippólito, Alicia G. and Bisca, Jacqueline M. and Hirot, Inajara N. and Teixeira, Carlos R. (2019) Production and evaluation of leukocyte- and thrombocyte-rich fibrin membranes in birds. Acta Veterinaria Hungarica, 67 (2). pp. 296-306. ISSN 0236-6290

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Abstract

The aim of this study was the preparation and histological evaluation of Leukocyte- and Thrombocyte-Rich Fibrin (L-TRF) membranes obtained from the blood of four bird species. Forty adult healthy birds were divided into four groups of equal size: G1 – macaws, G2 – domestic chickens, G3 – parrots, G4 – toco toucans. A total of 0.5 mL of blood was collected from each bird, put into a glass tube without anticoagulant and centrifuged at 3000 rpm for 10 min. L-TRF membranes produced after compression of the clot were processed for histological analysis. The ratio of thrombocytes/area was not significantly different among Groups G2, G3 and G4, but a significant difference was found between Groups G1 and G2 with the highest thrombocyte concentration/area in G1. The groups did not differ statistically in the number of leukocytes/area. The fibrin-to-cells ratio did not vary statistically among Groups G1, G2 and G3, but this ratio was significantly higher in Group G4 than in the other groups. The thrombocyte-to-leukocyte ratio was the highest in Group G1, but it did not differ among Groups G2, G3 and G4. In conclusion, the centrifugation protocol allowed the production of L-TRF membranes in the four bird species studied. Histologically, cell ratios were analogous in domestic chickens and parrots, and macaws had the highest ratio of thrombocytes.

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