REAL

Stimulating brain recovery after stroke using theranostic albumin nanocarriers loaded with nerve growth factor in combination therapy

Feczkó, Tivadar and Piiper, Albrecht and Ansar, Saema and Blixt, Frank and Ashtikar, Mukul and Schiffmann, Susanne and Ulshöfer, Thomas and Parnham, Michael and Harel, Yifat and Israel, Liron and Lellouche, Jean-Paul and Wacker, Matthias (2019) Stimulating brain recovery after stroke using theranostic albumin nanocarriers loaded with nerve growth factor in combination therapy. JOURNAL OF CONTROLLED RELEASE, 293. pp. 63-72. ISSN 0168-3659

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Abstract

For many years, delivering drug molecules across the blood brain barrier has been a major challenge. The neuropeptide nerve growth factor is involved in the regulation of growth and differentiation of cholinergic neurons and holds great potential in the treatment of stroke. However, as with many other compounds, the biomolecule is not able to enter the central nervous system. In the present study, nerve growth factor and ultrasmall particles of iron oxide were co-encapsulated into a chemically crosslinked albumin nanocarrier matrix which was modified on the surface with apolipoprotein E. These biodegradable nanoparticles with a size of 212 ± 1 nm exhibited monodisperse size distribution and low toxicity. They delivered NGF through an artificial blood brain barrier and were able to induce neurite outgrowth in PC12 cells in vitro. In an animal model of stroke, the infarct size was significantly reduced compared to the vehicle control. The combination therapy of NGF and the small-molecular MEK inhibitor U0126 showed a slight but not significant difference compared to U0126 alone. However, further in vivo evidence suggests that successful delivery of the neuropeptide is possible as well as the synergism between those two treatments.

Item Type: Article
Subjects: R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan
Depositing User: Dr. Tivadar Feczkó
Date Deposited: 10 Sep 2019 13:06
Last Modified: 10 Sep 2019 13:06
URI: http://real.mtak.hu/id/eprint/98945

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