Transformation of Revertant Murine Cells by 5-Azacytidine Results in Rapid Inhibition of Lysyl Oxidase Expression

Tze-Jou, Annie Yeh and Contente, Sara and Friedman, R. M. (2005) Transformation of Revertant Murine Cells by 5-Azacytidine Results in Rapid Inhibition of Lysyl Oxidase Expression. Acta Microbiologica et Immunologica Hungarica, 52 (3-4). pp. 433-442. ISSN 1217-8950

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Abstract

Lysyl oxidase (LO) is synthesized intracellularly as a proenzyme that is secreted and then processed extracellularly to a mature form. LO is expressed in NIH3T3 cells, but only very low levels are observed after NIH 3T3 is transformed by c-H-ras or one of several other oncogenes. LO functions as a tumor suppressor. Treatment of ras-transformed cells with interferon-a with or without retinoic acid results in their persistent reversion to a non-transformed state that is dependent on the restoration of LO expression. When such revertant cells are treated with 5-azacytidine (5-azaC), they undergo rapid morphological retransformation. Within one passage after addition of 5-azaC, there was a down regulation of LO mRNA and proenzyme protein. These data suggest a direct relationship between the transformed state and LO expression.

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