Regular transcutaneous myostimulation alters skeletal muscle phospholipid fatty acid composition and oxidative stability in rabbits

Szabó, A. and Fébel, H. and Mézes, M. and Szendrő, Z. and Miklós, S. and Romvári, R. (2005) Regular transcutaneous myostimulation alters skeletal muscle phospholipid fatty acid composition and oxidative stability in rabbits. Acta Physiologica Hungarica, 92 (3-4). pp. 193-202. ISSN 0231-424X

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Abstract

This experiment was performed to study the effects of regular, isometric, electrically induced contractions on skeletal muscle phospholipid (PL) and triglyceride (TG) fatty acid profile and oxidative stability. Transcutaneous electrical myostimulation of lo ngissimus dorsi (30 Hz, 20 ms impulse length, 10 mA, for 2×20 min daily) was performed on 8 Pannon White male rabbits for 4 weeks; 8 animals were set as control. Muscle TG and PL fatty acid profiles were determined by capillary gas chromatography after lip id fractionation. In the PL, the proportion of pentadecanoic (C15:0), palmitic (C16:0), linoleic (C18:2 n-6) and γ-linolenic (C18:3 n-6) acids decreased significantly compared to the control, as well as the sum of total saturated fatty acids. Increasing pr oportions of palmitoleic (C16:1n-7), gondoic (C20:1 n-9), arachidonic (C20:4 n-6), eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids were measured. The C20:4 n6/C20:3 n-6 ratio (estimated ∆5 desaturase activity), the sum of polyunsaturat ed fatty acids (PUFA) of 20 to 22 carbon chain length, the sum of total n-6 fatty acids and the unsaturation index increased. In the TG, stearic acid (C18:0) proportion was significantly decreased by the stimulation. Electrical treatment increased oxidative stability as assessed by thiobarbituric acid reactive substances (TBARS).

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