REAL

Penetration of lycopsamine from a comfrey ointment through human epidermis

Jedlinszki, Nikoletta and Balázs, Boglárka and Csányi, Erzsébet and Csupor, Dezső (2017) Penetration of lycopsamine from a comfrey ointment through human epidermis. REGULATORY TOXICOLOGY AND PHARMACOLOGY, 83. pp. 1-4. ISSN 0273-2300

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Abstract

Abstract Mutagenic and teratogenic pyrrolizidine alkaloids (PAs) have been identified in several plant species. The industrially most important PA-containing plant is Symphytum officinale (common comfrey). The application of its root is restricted in several countries due to its PA content. In medicines, the daily alkaloid quantity and duration of treatment may be limited even in case of topical application. Due to the confirmed good absorption of PAs from the gastrointestinal tract, the prohibition of oral use is justified, however the limitation of external application is not supported by relevant data. Penetration experiments on human skin are not available to be a rational basis for limitation. The aim of our work was to carry out pharmacokinetic studies on the diffusion and penetration of lycopsamine (a main PA of comfrey) from a Symphytum product through a synthetic membrane and human skin. Investigations were carried out on vertical Franz diffusion cell and lycopsamine was quantified by a validated LC-MS method. The amount of lycopsamine diffused through a synthetic membrane varied between 0.11% and 0.72% (within 24 h). On human epidermis, the rate of penetration was lower (0.04–0.22%). Our results may contribute to the more realistic toxicological assessment of externally applied PA-containing products.

Item Type: Article
Uncontrolled Keywords: ABSORPTION; SKIN; Epidermis; pyrrolizidine alkaloid; Franz diffusion cell; Comfrey; Lycopsamine;
Subjects: R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 24 Sep 2019 07:37
Last Modified: 24 Sep 2019 07:37
URI: http://real.mtak.hu/id/eprint/100728

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