Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley

Gierczik, Krisztián and Székely, András and Ahres, Mohamed and Marozsán-Tóth, Zsuzsa and Vashegyi, Ildikó and Harwood, Wendy and Tóth, Balázs and Galiba, Gábor and Soltész, Alexandra and Vágújfalvi, Attila (2019) Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley. PLANT MOLECULAR BIOLOGY REPORTER, 37 (4). pp. 314-326. ISSN 0735-9640 (Unpublished)

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Abstract

Phosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4 kinase (PI4K) are upstream regulatory elements of the phospholipid signaling pathway. PITP and PI4K over expressing transgenic barley lines were developed and studied. We revealed that the over expression of the PITP and PI4K genes increased stress tolerance during hypoxic cold stress, but not during salinity stress, and differences were also found in the level of frost tolerance. On the other hand, we ralised that the over expression of these upstream signaling elements led to more phenotypic abnormalities than in other transgenic studies working with effector genes or even transcription factors. We hypothesize that this high level of abnormalities are the consequence of the modulation of a very upstream signal transduction pathway elements.

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