Synthesis and spectroscopic characterization of novel GFP chromophore analogues based on aminoimidazolone derivatives

Jancsó, Attila and Kovács, Ervin and Cseri, Levente and Rózsa, Balázs and Galbács, Gábor and Csizmadia, Imre Gy. and Mucsi, Zoltán (2019) Synthesis and spectroscopic characterization of novel GFP chromophore analogues based on aminoimidazolone derivatives. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 218. pp. 161-170. ISSN 1386-1425

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Abstract

In order to improve the fluorescence properties of the green fluorescent protein chromophore, p‑HOBDI ((5‑(4‑hydroxybenzylidene)‑2,3‑dimethyl‑3,5‑dihydro‑4H‑imidazol‑4‑one), sixteen dihydroimidazolone derivates were synthesized from thiohydantoin and arylaldehydes. The synthesis developed is an efficient, novel, one-pot procedure. The study provides a detailed description of the spectroscopic characteristics of the newly synthesized compounds, using p‑HOBDI as a reference. The new compounds all exhibited significantly stronger fluorescence than p‑HOBDI, up to 28 times higher quantum yields. An experimental and theoretical investigation of the relationship of the fluorescence properties with the molecular structure was also carried out. A good correlation was found between the emission wavenumber and the Hammett constant of the functional group, which suggests the intermolecular charge transfer (ICT) mechanism between the aromatic groups.

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