Interaction Force Fluctuations in Antigen-Antibody Biorecognition Studied by Atomic Force Spectroscopy

Bizzarri, Anna Rita and Végh, Attila Gergely and Váró, György and Cannistraro, Salvatore (2019) Interaction Force Fluctuations in Antigen-Antibody Biorecognition Studied by Atomic Force Spectroscopy. ACS OMEGA, 4 (2). pp. 3627-3634. ISSN 2470-1343

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Abstract

The formation of a specific complex between beta(2)mu globulin and anti beta(2)mu globulin was investigated by analyzing the force fluctuations recorded in an atomic force spectroscopy biorecognition experiment. We found that a 1/f noise appears in the power spectra of force fluctuations when the tip, functionalized with beta(2)mu globulin, reaches a distance of 0.50 nm from the partner-charged substrate while a specific biorecognition process occurs. Concomitantly, in this active region, the distribution of the times spent by the tip in the proximity of the substrate exhibits a power law trend characterized by a longtime tail. All of these findings are put into relationship to a slowing down of the energy landscape exploration, consistent with a restricted sampling dynamics of the conformational states driving to the final binding state. The hypothesis that a combination of a conformational substrate and an induced fit hybrid binding mechanism controls the specific complex formation is put forward and discussed also in connection with the fluctuations of the hydration water network.

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