Boda, Attila and Lőrincz, Péter and Takáts, Szabolcs and Csizmadia, Tamás and Tóth, Sarolta and Kovács, Attila Lajos and Juhász, Gábor (2019) Drosophila Arl8 is a general positive regulator of lysosomal fusion events. BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR CELL RESEARCH, 1866 (4). pp. 533-544. ISSN 0167-4889
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Abstract
The small GTPase Ar18 is known to be involved in the periphery-directed motility of lysosomes. However, the overall importance of moving these vesicles is still poorly understood. Here we show that Drosophila Ar18 is required not only for the proper distribution of lysosomes, but also for autophagosome-lysosome fusion in starved fat cells, endosome-lysosome fusion in garland nephrocytes, and developmentally programmed secretory granule degradation (crinophagy) in salivary gland cells. Moreover, proper Ar18 localization to lysosomes depends on the shared subunits of the BLOC-1 and BORC complexes, which also promote autophagy and crinophagy. In conclusion, we demonstrate that Ar18 is responsible not only for positioning lysosomes but also acts as a general lysosomal fusion factor.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Hungarian Academy of Sciences [LP-2014/2, PPD-003/2016, PPD-222/2018]; National Research, Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00006, -00032, K119842, KH125108]; UNKP New National Excellence Program of the Ministry of Human Capacities of Hungary [UNKP-17-3-I-ELTE-27, UNKP-18-3-I-ELTE-314] Funding text: This work was funded by the Hungarian Academy of Sciences (LP-2014/2 to GJ; PPD-003/2016 to ST; PPD-222/2018 to PL), the National Research, Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00006 and -00032, K119842 to GJ and KH125108 to ST) and the UNKP New National Excellence Program of the Ministry of Human Capacities of Hungary (UNKP-17-3-I-ELTE-27 and UNKP-18-3-I-ELTE-314 to BA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Összes idézések száma a WoS-ban: 0 Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Hungarian Academy of Sciences, Premium Postdoctoral Research Program, Budapest, Hungary Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :1 Export Date: 9 September 2019 CODEN: BAMRD Correspondence Address: Juhász, G.; Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd UniversityHungary; email: szmrt@elte.hu Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Hungarian Academy of Sciences, Premium Postdoctoral Research Program, Budapest, Hungary Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :2 Export Date: 18 October 2019 CODEN: BAMRD Correspondence Address: Juhász, G.; Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd UniversityHungary; email: szmrt@elte.hu Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Hungarian Academy of Sciences, Premium Postdoctoral Research Program, Budapest, Hungary Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :2 Export Date: 21 October 2019 CODEN: BAMRD Correspondence Address: Juhász, G.; Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd UniversityHungary; email: szmrt@elte.hu Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Hungarian Academy of Sciences, Premium Postdoctoral Research Program, Budapest, Hungary Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary Cited By :2 Export Date: 22 October 2019 CODEN: BAMRD Correspondence Address: Juhász, G.; Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd UniversityHungary; email: szmrt@elte.hu |
| Uncontrolled Keywords: | PROTEINS; TRANSPORT; FUSION; Autophagy; endosome; SPATIAL-DISTRIBUTION; HOPS COMPLEX; Biochemistry & Molecular Biology; Crinophagy; Ar18; ARF-LIKE GTPASE; RAB7 EFFECTOR; SALIVARY-GLAND; ARL8B; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 21 Nov 2019 08:22 |
| Last Modified: | 21 Nov 2019 08:22 |
| URI: | http://real.mtak.hu/id/eprint/103443 |
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