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mmSTORM: Multimodal localization based super-resolution microscopy

Gajdos, Tamás and Cserteg, Zsófia and Szikora, Szilárd and Novák, Tibor and Kovács, Bálint Barna H. and Szabó, Gábor and Mihály, József and Erdélyi, Miklós (2019) mmSTORM: Multimodal localization based super-resolution microscopy. SCIENTIFIC REPORTS, 9. ISSN 2045-2322

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Abstract

Super-resolution localization microscopy provides a powerful tool to study biochemical mechanisms at single molecule level. Although the lateral position of the fluorescent dye molecules can be determined routinely with high precision, measurement of other modalities such as 3D and multicolor without the degradation of the original super-resolved image is still in the focus. In this paper a dual-objective multimodal single molecule localization microscopy (SMLM) technique has been developed, optimized and tested. The proposed optical arrangement can be implemented onto a conventional inverted microscope without serious system modification. The performance of the method was tested using fluorescence beads, F-actin filaments and sarcomere structures. It was shown that the proposed imaging method does not degrade the image quality of the original SMLM 2D image but could provide information on the axial position or emission spectra of the dye molecules.

Item Type: Article
Uncontrolled Keywords: RESOLUTION; Orientation; TRACKING; accuracy; SINGLE MOLECULES; 3-DIMENSIONAL SUPERRESOLUTION; FLUORESCENCE NANOSCOPY; PARTICLE LOCALIZATION; DIFFRACTION-LIMIT;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 21 Nov 2019 11:01
Last Modified: 21 Nov 2019 11:01
URI: http://real.mtak.hu/id/eprint/103519

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