Nemestóthy, Nándor and Megyeri, Gábor and Bakonyi, Péter and Lakatos, Patrik and Koók, László and Polakovic, Milan and Gubicza, László and Bélafi-Bakó, Katalin (2017) Enzyme kinetics approach to assess biocatalyst inhibition and deactivation caused by [bmim][Cl] ionic liquid during cellulose hydrolysis. BIORESOURCE TECHNOLOGY, 229. pp. 190-195. ISSN 0960-8524 (Unpublished)
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Enzyme kinetics approach to assess biocatalyst inhibition and deactivation caused by [bmim][Cl] ionic liquid during cellulose hydrolysis.pdf Download (1MB) | Preview |
Abstract
The aim of this work was to study the inhibition and deactivation of commercial enzyme cocktail (Cellic® Htec2) in the presence of [bmim][Cl] ionic liquid employing model cellulosic substrate, carboxymethyl cellulose (CMC). It turned out from the experiements – relying on enzyme kinetics appproach – that [bmim][Cl] could act as a competitive inhibitor. Furthermore, depending on the process conditions i.e. contact of enzyme solution with high concentration [bmim][Cl], severe biocatalyst inactivation should be also taken into account as a potential risk during the enzymatic cellulose hydrolysis even in as short process times as few minutes.
| Item Type: | Article |
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| Subjects: | T Technology / alkalmazott, műszaki tudományok > TD Environmental technology. Sanitary engineering / környezetvédelem, hulladékkezelés, egészségügyi mérnöki technika (ivóvízellátási és szennyvízkezelési technika) |
| Depositing User: | Péter Bakonyi |
| Date Deposited: | 06 Feb 2020 08:13 |
| Last Modified: | 06 Feb 2020 08:13 |
| URI: | http://real.mtak.hu/id/eprint/106155 |
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