Mahmoudi, H. and Salari, M. and Ghayeb Zamharir, M. and Ghorbani, M. (2019) Molecular study of a phytoplasma associated with safflower fasciation in Iran. Acta Phytopathologica et Entomologica Hungarica, 54 (2). pp. 203-209. ISSN 0238-1249
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Abstract
A safflower disease was observed in the fields of South Khorasan (Iran) in 2017–2018. Affected plants had extensive fasciation, bushy growth, small leaves, phyllody and shortened internodes. Polymerase chain reaction (PCR) assays were performed using universal phytoplasma primers pairs P1/P7, R16mF1/mR1 and M1/M2 to detect putative phytoplasma (s). Nested PCR analyses showed that all symptomatic plants were positive for phytoplasma infection, while asymptomatic plants were negative. Nucleotide sequence analysis and RFLP analyses of PCR-amplified 16S rDNA sequences indicated that safflower fasciation in Iran was associated with a phytoplasma that belonged to the peanut witches' broom group (16SrII). Phylogenetic analysis confirmed that safflowers fasciation phytoplasma (SaP) must be classified in ‘Candidatus phytoplasma aurantifolia’ (16SrII) taxonomic group. To our knowledge, this is the first documentation of a phytoplasma associated with safflower fasciation in Iran and the world.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH540 Ecology / ökológia Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan Q Science / természettudomány > QL Zoology / állattan > QL01 Systematic zoology / állatrendszertan |
| Depositing User: | Ágnes Sallai |
| Date Deposited: | 28 Feb 2020 07:54 |
| Last Modified: | 31 Dec 2020 00:44 |
| URI: | http://real.mtak.hu/id/eprint/106759 |
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