Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases

Debreczeni, Márta Lídia and Székács, Inna and Kovács, Boglárka and Saftics, András and Kurunczi, Sándor and Gál, Péter and Dobó, József and Cervenák, László and Horváth, Róbert (2020) Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases. SCIENTIFIC REPORTS, 10. pp. 1-14. ISSN 2045-2322

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Tissue-on-a-chip technologies are more and more important in the investigation of cellular function and in the development of novel drugs by allowing the direct screening of substances on human cells. Constituting the inner lining of vessel walls, endothelial cells are the key players in various physiological processes, moreover, they are the first to be exposed to most drugs currently used. However, to date, there is still no appropriate technology for the label-free, real-time and high-throughput monitoring of endothelial function. To this end, we developed an optical biosensor-based endothelial label-free biochip (EnLaB) assay that meets all the above requirements. Using our EnLaB platform, we screened a set of plasma serine proteases as possible endothelial cell activators, and first identified the endothelial cell activating function of three important serine proteases – namely kallikrein, C1r and mannan-binding lectin-associated serine-protease 2 (MASP-2) – and verified these results in well-established functional assays. EnLaB proved to be an effective tool for revealing novel cellular mechanisms as well as for the high-throughput screening of various compounds on endothelial cells.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Depositing User: MTMT SWORD
Date Deposited: 28 Feb 2020 09:43
Last Modified: 28 Feb 2020 09:43

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