Studies on molecular interactions between nalidixic acid and liposomes

Budai, Marianna and Szabó, Zs. and Zimmer, A. and Szőgyi, M. and Gróf, Pál (2004) Studies on molecular interactions between nalidixic acid and liposomes. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 279. pp. 67-79. ISSN 0378-5173

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The interaction between nalidixic acid sodium salt (NANa) and liposomes prepared from alfa-l-dipalmitoyl-phosphatidylcholine (DPPC) or from its binary mixture with dioleoyl-phosphatidylcholine (DOPC)was studied with differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) spectroscopy. We evaluated the role of broadband ultraviolet-B (UV-B) irradiation on the molecular interactions between the lipids and the NANa, and determined the decay-kinetics of the incorporated spin labeled fatty-acid free radicals. Multilamellar and unilamellar vesicles were prepared by sonication and extrusion. The entrapment efficiencies were determined spectrophotometrically. The size-distribution of the liposomes and its change in time was checked by dynamic light scattering (DLS). Our results indicate thatNANa mainly interacts with lipid head groups. However, its effect and presumably the formation of the free radicals, induced by broadband ultraviolet-B, is not localized only to the head group region of the lipid molecules. Depending on DOPC content, interaction between the NANa and the lipids modifies the phase-transition parameters of the liposome dispersions.

Item Type: Article
Uncontrolled Keywords: Liposomes; Nalidixic acid; DSC; DLS; Zeta-potential; EPR
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Depositing User: MTMT SWORD
Date Deposited: 24 Apr 2014 21:14
Last Modified: 26 Apr 2014 05:44

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