Interaction of photosensitizers with liposomes containing unsaturated lipid

Voszka, István and Budai, Marianna and Szabó, Zsófia and Maillard, Philip and Csik, Gabriella and Gróf, Pál (2007) Interaction of photosensitizers with liposomes containing unsaturated lipid. CHEMISTRY AND PHYSICS OF LIPIDS, 145 (2). pp. 63-71. ISSN 0009-3084

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Small unilamellar liposomes were made of dipalmitoyl-phosphatidylcholine and dioleoyl-phosphatidylcholine, and photosensitized by a symmetrically or an asymmetrically substituted glycosilated tetraphenyl-porphyrin derivative. As differential scanning calorimetry and electron paramagnetic resonance spectroscopy (EPR) revealed these porphyrin derivatives were localized in different depth within the lipid bilayer. Both porphyrin derivatives were able to induce photoreaction and consequent structural changes in the membrane. 5-, 12-, or 16-doxyl stearic acid labeled lipid bilayers were applied and the efficiency of photoinduced reaction was followed by the decay of their EPR signal amplitude. Light dose-dependent destruction of nitroxide radical proved to be dependent on the position of spin label. In this process the porphyrin localized in closer connection with the double bond of unsaturated fatty acid was more effective. EPR signal decay was also dependent on the unsaturated fatty acid content of the liposome and the oxygen saturation of the solvent.

Item Type: Article
Uncontrolled Keywords: Photosensitizer; Porphyrin; Liposomes; Electron paramagnetic resonance; Spin labeling; Differential scanning calorimetry
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Depositing User: MTMT SWORD
Date Deposited: 25 Apr 2014 08:36
Last Modified: 25 Apr 2014 08:36

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